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bio rad pfge tank  (Bio-Rad)


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    Bio-Rad bio rad pfge tank
    Bio Rad Pfge Tank, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio rad pfge tank/product/Bio-Rad
    Average 93 stars, based on 23 article reviews
    bio rad pfge tank - by Bioz Stars, 2026-04
    93/100 stars

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    bio rad pulsed field gel electrophoresis pfge tank  (Bio-Rad)
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    Figure 4. (A) Formation of stable cell lines expressing HVS-GFP-BAC or HVS-GFP-APC5. SW480 cells were infected with HVS-GFP-BAC or HVS- GFP-BAC-APC5 viruses and then expanded through routine tissue culture in the presence of hygromycin (B) in order to remove any non-fluorescing cells. GFP fluorescence was observed in these stable cell lines throughout the 8-week time course. (B) Episome rescue studies were performed on SW480HVSAPC cells and analysed via I-ppo-I restriction digest and <t>PFGE.</t> These results show that the vectors were stably maintained in an episomal state for 8 weeks. However this does completely rule out the possibility of some level of genome integration.
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    Figure 4. (A) Formation of stable cell lines expressing HVS-GFP-BAC or HVS-GFP-APC5. SW480 cells were infected with HVS-GFP-BAC or HVS- GFP-BAC-APC5 viruses and then expanded through routine tissue culture in the presence of hygromycin (B) in order to remove any non-fluorescing cells. GFP fluorescence was observed in these stable cell lines throughout the 8-week time course. (B) Episome rescue studies were performed on SW480HVSAPC cells and analysed via I-ppo-I restriction digest and PFGE. These results show that the vectors were stably maintained in an episomal state for 8 weeks. However this does completely rule out the possibility of some level of genome integration.

    Journal: International journal of oncology

    Article Title: Herpesvirus saimiri-mediated delivery of the adenomatous polyposis coli tumour suppressor gene reduces proliferation of colorectal cancer cells.

    doi: 10.3892/ijo.2011.1130

    Figure Lengend Snippet: Figure 4. (A) Formation of stable cell lines expressing HVS-GFP-BAC or HVS-GFP-APC5. SW480 cells were infected with HVS-GFP-BAC or HVS- GFP-BAC-APC5 viruses and then expanded through routine tissue culture in the presence of hygromycin (B) in order to remove any non-fluorescing cells. GFP fluorescence was observed in these stable cell lines throughout the 8-week time course. (B) Episome rescue studies were performed on SW480HVSAPC cells and analysed via I-ppo-I restriction digest and PFGE. These results show that the vectors were stably maintained in an episomal state for 8 weeks. However this does completely rule out the possibility of some level of genome integration.

    Article Snippet: A Bio-Rad pulsed field gel electrophoresis (PFGE) tank was filled with 2.5 l, 0.5X TBE buffer (0.0225 M Tris-borate, 0.001 M EDTA).

    Techniques: Stable Transfection, Expressing, Infection, Fluorescence